摘要
目的建立山东不同品种玫瑰花药材的过氧化物酶同工酶鉴别方法。方法聚丙烯酰胺凝胶电泳法。结果19个品种的玫瑰花过氧化物酶同工酶分析,显现出谱带1～5条,其中D1带和D2带是玫瑰的基本谱带。结论山东产玫瑰花各品种过氧化物酶同工酶的谱型较多,品种之间有较明显的差异,可作为玫瑰花栽培品种鉴别的依据,为玫瑰花的规范化栽培提供了资料。
Objective To establish a Peroxidase isoenzyme（POD） method for identification of mgosa rose varieties in Shandong province. Methods The polyacrylamide gel electrophoresis method was applied. Results Peroxidase zymograms of the 19 species were analyzed. The results showed that there were one to five belts, of which D1 and D2 were basic. Conclusion The significant difference of POD zymograms among rugosa rose varieties in Shandong can be used as a foundation, which will provide information for normalized cultivation.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第7期1624-1625,共2页
Lishizhen Medicine and Materia Medica Research
基金
山东省中医药科技计划项目(No.2005-062
2007-168)
山东省山东大学卫生局科技计划项目(No.2005-041)
关键词
玫瑰花
过氧化物酶同工酶
电泳
品种鉴别
Rugosa rose
Peroxidase isoenzyme
Electrophoresis
Variety identification
