摘要
目的建立柱前衍生化HPLC/UV法测定黄杨宁片中环维黄杨星D的含量。方法异氰酸苯酯为柱前衍生化试剂,流动相：甲醇-水（83∶17）;ZORBAX SB-C18（250 mm×4.6 mm,3.5μm）柱分离;检测波长242 nm;流速：1 mL.min-1;柱温：30℃;进样量：10μL。结果环维黄杨星D在2～400μg.mL-1与峰面积呈良好线性关系,回归方程为：Y=2 616X＋198.2（r=0.999 9）;环维黄杨星D平均回收率为102.6%,RSD为0.4%（n=6）;结论该法简单、准确度高、灵敏度好,为黄杨宁片质量控制提供了依据。
Objective To establish a pre-column derivatization of HPLC/UV method for the content determination of cyclovirobuxine D in Huangyangning tablets. Methods A pre-column derivation was developed by HPLC with phenylisocyanate. A ZORBAX SB-Cx8 column （250 min×4. 6 mm, 3. 5μm） was used and the mobile phase contained a mixture of methanol-water （83 ： 17）, with a flow rate at 1.0 mL ·min-1. The column temperature was 30℃. The UV detection wavelength was 242 nm. Results The calibration curve of cyclovirobuxine D was linear at 2～400μg·mL （r=0. 999 9）. The average recovery was 102.6%, with RSD of 0. 4% （n=6）. Conclusion The method is simple, accurate, sensitive and suitable for the quality control of Huangyangning tablets.
出处
《中南药学》
CAS
2009年第7期484-486,共3页
Central South Pharmacy
基金
深圳市科技计划项目(医疗卫生类)
(编号:200802087)
