摘要
目的采用HPLC测定复方甘草酸苷片中甘草酸单铵的溶出度。方法采用Kromasil 100A C18柱(250mm×4.6mm,5μm),以2%的醋酸-乙腈(65:35)为流动相;检测波长为254nm;流速为1.0mL·min-1。结果甘草酸单铵在80～800μg·mL-1范围内有良好的线性关系(r=0.9999),平均加样回收率为98.74%,RSD为0.9%。结论该法简便可靠,精密度高,分离度好,可用于复方甘草酸苷片的溶出度测定。
OBJECTIVE To develop an HPLC method for the determination of dissolution of compound glycyrrhizin tablets. METHODS The analytical column was a shim-pack Kromasil 100A Cl8 （250 mm×4.6 ram, 5 μm）. The mobile phase consisted of 2% acetic acid solution-acetonitrile （35：65）. The detection wavelength was 254 nm, and the flow-rate was 1.0 mL·min^-1. RESULTS Ammonium glycyrrhizinate showed good linear relationship at the range of 80-800 μg·mL^-1（r=0.999 9）. The average recovery was 98.74%, RSD 0.9%. CONCLUSION The results showed that this method was reliable and accurate, and can be used for dissolution of compound glycyrrhizin tablets.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第7期598-601,共4页
Chinese Journal of Modern Applied Pharmacy
