摘要
目的:观察桃仁、木香、黄芪分别与红花配伍对成年SD大鼠早期桡骨骨折愈合过程中骨痂厚度及VEGF表达的影响,为理气活血法用于四肢闭合性骨折早期临床治疗提供实验支持。方法:256只SD大鼠随机分为模型组、桃仁红花组、木香红花组、黄芪红花组4组,每组各64只,各组大鼠均造成左侧桡骨骨折模型。于造模后第3天、第7天、第10天、第14天,各组大鼠分4次处死,每次每组处死16只,取材进行常规HE染色观察骨痂厚度,VEGF-DAB显色计数观察VEGF阳性细胞数。结果:造模后第3天,各给药组与模型组比较,标本VEGF阳性细胞数及骨痂厚度差异无统计学意义(P>0.05);造模后第7天、第10天和第14天,各给药组与模型组比较,标本VEGF阳性细胞数及骨痂厚度差异有统计学意义(P<0.05,P<0.01),桃仁红花组、黄芪红花组与木香红花组比较,标本VEGF阳性细胞数及骨痂厚度差异有统计学意义(P<0.05)。结论:木香、红花配伍能显著提高SD大鼠早期桡骨骨折断端局部VEGF的表达水平,增加骨痂厚度,提示理气活血法是四肢闭合骨折早期有效内治法。
Objective：To observe the effect of different compatibility of herbs on VEGF expression at fracture site and thickness of callus of early radius fracture rat models. Methods：256 SD rats were divided into four groups randomly, model group,Semen Persicae and Flos Carthami group, Radix Aucklandiae and Flos Carthami group, Radix Astragali and Flos Carthami group.Left radius fracture rat models were established. Conventional haematoxylin and eosin staining and immunostaining for bone section were carried out on days 3,7,10 and 14 after model establishment. Results ：On days 7,10 and 14 after model establishment,compared with model group,VEGF positive cells at fracture site and thickness of callus were significantly increased in all the treatment groups;compared with Radix Aucklandiae and Flos Carthami group,VEGF positive cells at fracture site and thickness of callus were significantly decreased in Semen Persicae and Flos Carthami group,Radix Astragali and Flos Carthami group. Conclusions：Radix Aucklandiae and Flos Carthami can increase obviously VEGF expression at fracture site and thickness of callus in the healing of early radius fracture rats.We can get a conclusion that the therapy of regulating the flow of qi and promoting the circulation of blood is an effective therapy on early extremities closed fracture from this experiment.
出处
《山西中医学院学报》
2009年第3期18-20,共3页
Journal of Shanxi College of Traditional Chinese Medicine
基金
山西省卫生厅科技攻关计划项目(200838)
关键词
桡骨骨折
VEGF
免疫组化
radius fracture
VEGF
immunohistochemistry
