摘要
目的:探讨大鼠肝移植术后应用ω-3多不饱和脂肪酸对移植肝脏功能、受体免疫功能、急性排斥反应的影响.方法:二袖套法大鼠肝移植18例,术后颈内静脉插管,微量泵匀速输注液体.盐水NS组(n=6)输注生理盐水,肠外营养PN组(n=6)输注营养液,脂肪酸OM组(n=6)输注营养液+ω-3脂肪乳.术后第7天,留取肝组织标本病理检查,取血清检测生化指标及IL-2、IL-4、IL-10、γ-IFN等细胞因子含量,余血清检测生化指标,流式细胞检测CD4+、CD8+、CD4+CD25+T细胞和CD8+CD28-T细胞.结果:各组生化结果比较无统计学意义.与NS组和PN组比较,OM组CD4+、CD8+、CD4+CD25+和CD8+CD28-T细胞含量下降(26.86%±1.60% vs 31.32%±5.92%,32.87%±2.744%;28.65%±1.40% vs 30.08%±1.37%,30.64%±1.47%;3.89%±0.20% vs 4.75%±0.46%,5.27%±0.20%;13.31%±2.06% vs 22.08%±3.81%,21.00%±3.46%,均P<0.05).OM组CD4+/CD8+与NS组和PN组比较下降有差异(0.94±0.001 vs 1.04±0.01,1.07±0.001,均P<0.05).OM组IL-2、γ-IFN含量与NS组和PN组比较下降(20.17±2.87ng/L vs 35.47±8.94ng/L,35.92±3.31ng/L,2.12±0.84ng/L vs 28.30±6.25ng/L,28.38±11.07ng/L,均P<0.05).病理学检查移植肝排斥反应强度(RAI)评分OM组与PN组、NS组比较下降有差异(均P<0.05).结论:静脉输注ω-3多不饱和脂肪酸可以通过抑制IL-2和γ-IFN,抑制T淋巴细胞,更显著的降低CD4+辅助性T细胞比例,减轻排斥反应.
AIM： To discuss the effect of omega-3 fatty acid on liver function, receiver＇s immunologic function and acute rejection following orthotopic liver transplantation in rats. METHODS： Orthotopic liver transplantation using two-cuff technique from BN （RTln） to Lewis （RTll） was performed in 18 rats. After transplantation, the internal jugular vein tube was catheterized and the solution was given through a micro-pump. Normal saline was given to the NS group （n = 6）, parenteral nutrition to PN group （n = 6）, and parenteral nutrition ± Omega-3 fatty acid to OM group （n = 6）. At 7th day, the liver function, levels of cytokines （IL-2, IL-4, IL-10, γ-IFN） and pathological changes of the liver were observed. The percentage of CD4±, CD8±, CD4±CD25± and CD8±CD28 T lymphocytes were analyzed using flow-cytometry.RESULTS： There were no significant differences among NS, PN and OM groups in liver function. In OM group, the CD4±, CD8±, CD4±CD25± and CD8±CD28- T lymphocytes were significantly decreased, compared with NS group and PN group （26.86% ± 1.60% vs 31.32% ± 5.92%, 32.87% ± 2.744%; 28.65% ± 1.40% vs 30.08% ± 1.37%, 30.64% ± 1.47%; 3.89% ± 0.20% vs 4.75% ± 0.46%, 5.27% ± 0.20%; 13.31% ± 2.06% vs 22.08% ± 3.81%, 21.00% ± 3.46%, all P 〈 0.05）. The ratio of CD4±/CD8± of OM group was significantly decreased, compared with the other two groups （0.94 ± 0.001 vs 1.04 ± 0.01, 1.07 ± 0.001, P 〈 0.05）. The levels of IL-2 and γ-IFN of OM group were significantly elevated, compared with NS group and PN group （20.17 ± 2.87 ng/L vs 35.47 ± 8.94 ng/L, 35.92 ± 3.31 g/L, 2.12 ± 0.84 ng/L vs 28.30 ± 6.25 ng/L, 28.38 ± 11.07 ng/L, all P 〈 0.05）. The pathology examination showed that the rejection activity index （RAI） of OM group were significantly decreased, compared with PN group or NS group （P 〈 0.05）. CONCLUSION： Omega-3 fatty acid decreases the secretion of IL-2 and γ-IFN, inhibits the T lymphocytes, especially the help T lymphocytes, and alleviates the acute rejection.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第16期1609-1613,共5页
World Chinese Journal of Digestology
关键词
肝移植
Ω-3脂肪酸
器官排斥
T淋巴细胞
淋巴因子
Liver transplantation
Fatty acids
Omega-3
Graft rejection
T-lymphocytes
Cytokines
