摘要
目的:研究广西肝癌高发区和低发区人群中CYP3A5基因多态性,以探讨CYP3A5基因多态性与肝癌易感性的关系.方法:收集广西医科大学第一附属医院2005/12-2006/09经病理确诊的72例肝细胞癌(HCC)患者的肝癌组织及其癌旁组织,患者分别来自于肝癌高发区(黄曲霉素高污染区)和低发区.12例血管瘤的瘤旁肝组织作为正常对照.用PCR-RFLP法检测CYP3A5在上述标本中的多态性.结果:广西肝癌高发区患者CYP3A5*1等位基因频率和基因型比(CYP3A5*1/*1+CYP3A5*1/*3)与广西肝癌低发区患者比较,差异有统计学意义(P<0.05);肝癌高发区人群与正常对照组比较,差异有统计学意义(P<0.05);低发区与正常对照组之间比较,差异无统计学意义.结论:CYP3A5可能参与黄曲霉毒素B1(AFB1)相关性HCC的发生.
AIM： To investigate the relationship between genetic polymorphism of CYP3A5 and HCC affectability through analysis of two groups of patients coming from Guangxi HCC highincidence area and HCC low-incident area. METHODS： We had collected 72 cases of HCC tissues and HCC-adjacent tissues from the First Affiliated Hospital of Guangxi Medical University during 2005-12/2006-09, and all the cases, who came from Guangxi HCC high-incidence area and HCC low-incidence area respectively, had been pathologically confirmed. At the same time, we used 12 cases of hepatic angioma-surrounding tissue as the normal control group. PCR-RFLP assay was used to analyze CYP3A5 genetic polymorphism of the above-mentioned samples. RESULTS： There were statistic differences between the population with CYP3A5＊1 allele frequency and the proportion of （CY- P3A5＊1/＊1 ＋ CYP3A5＊1/＊3） genotypes of Guangxi HCC high-incidence area and the population of Guangxi HCC low-incidence area （P 〈 0.05）. There were statistic differenc- es between the population of Guangxi HCC high-incidence area and the population of the normal control group （P 〈 0.05）. There were no statistic differences between Guangxi HCC low-incidence area population and the normal control group. CONCLUSION： CYP3A5 might take part in the morbidity of AFB1 related HCC.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第15期1570-1574,共5页
World Chinese Journal of Digestology
