摘要
目的研究聚酰胺分离纯化独脚金总黄酮的工艺条件。方法以紫外可见分光光度法测定独脚金样品溶液中总黄酮的含量为指标,考察多个工艺参数。结果聚酰胺对独脚金的总黄酮有良好的吸附作用,其吸附分离工艺条件的药液浓度为1.12～2.24mg/mL,以2BV/h吸附速率进行吸附,95%乙醇250mL洗脱效果最佳。结论该方法简单易行,分离效果良好,适于独脚金中总黄酮的分离纯化。
Objective To study the technique condition for separating and purifying total flavones from Striga asiatica （L.） O. Ktze. by Polyamide. Methods Total flavones content, in the sample solution of Striga asiatica （L.） O. Ktze. , detected by UV spectrophotometry, is used as the index. Some technological parameters are observed by single factor observation. Results Polyamide has good absorbing effect on total flavones of Striga asiatica （L.） O. Ktze. The liquid concentration of its absorbing and separating technological condition is 1.12 - 2.24 mg/mL and at the absorbing speed of 2BV/h. The elution effect of 95 % alcohol of 250 mL is the best. Conclusion This method is simple and feasible, fit for separating and purifying total falvones from Striga asiatica （L.） O. Ktze.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第4期380-383,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省科技计划项目"木豆叶等6种岭南常用中草药质量标准化研究"(20065020104)
关键词
聚酰胺
独脚金
总黄酮
纯化分离
Polyamide
Striga asiatica （L.）O. Ktze.
Total falvones
Separation and purification
