摘要
试验采用琼脂平板扩散法(打孔法),研究了黄芩等21种中药对从乳房炎奶牛乳汁中分离出的3种主要致病菌的体外抑菌情况。结果发现,大肠杆菌对黄芩高度敏感,对丹参、青翘属中度敏感;金黄色葡萄球菌对黄芩、青翘、丹参、地榆、红花、蒲公英高度敏感,对枳壳、忍冬藤、萹蓄、苦地丁、蟾酥、木通中度敏感;无乳链球菌对地榆、当归属高度敏感,对青翘、黄芩、蒲公英、丹参、苦地丁、红花、川芎、枳壳中度敏感。为防治奶牛乳房炎有效中药复方的筛选提供了理论依据。
Three kinds of major pathogenic microorganisms that cause mastitis of diary cows were selected and the in vitro antibiosis effects of 21 kinds of Chinese medicinal herbs such as Radix Scutellariae et al. Were tested through the method of stiletto. Twenty four nutrient agar medium were divided into there groups. Smear Escherichia Coli on AltoA8 nutrient agar medium homogeneous. Smear S. aurous on B1 to B8 nutrient agar medium homogeneous. Smear Streptococcus agalactiae on CltoC8 nutrient agar medium homogeneous. Then slotting uniform upon the nutrient agar medium, respectively, each plate six hole. Add 20 μl of every medical solution into each hole, and two holes were made to parallel samples. Add 21 chinese medicine to AltoA7, BltoB7, CltoC7, respectively, and AS,B8,C8 were the normal control which were added deionized water only. These nutrient agar medium were incubated at 37 ℃ for 24 h. Bacterial existence and antibacterial circle diameter were observed. We found that different medicine for different strains produce different effects. The experiment results showed above supplied the scientific evidence for filtering Chinese medicinal herbs to prevent and cure the mastitis.
出处
《中国农学通报》
CSCD
北大核心
2009年第14期11-14,共4页
Chinese Agricultural Science Bulletin
基金
国家"十一五"科技支撑计划"华北农区高产奶牛良种繁育体系建立与奶业规模化生产技术研究-第四子课题-华北农区奶牛疾病综合防治技术应用与示范"(2006BAD04A10-4)
河北省重大技术创新专项计划项目"河北省奶业规模化生产技术集成与产业化示范-第四子课题-奶牛疾病综合防治技术的研究与开发"(07227146Z-4)
关键词
中药
奶牛
乳房炎
病原菌
抑杀效果
chinese herbal medicine, cow, mastitis, pathogenic bacteria, bacteriostasis effects in vitro
