摘要
红螺菌科光合细菌液体培养基由乙酸钠、氯化铵、磷酸二氢钾、硫酸镁、酵母膏等5种原料组成。采用L16(45)正交表,得到液体培养基的优化配方为:乙酸钠3.3 g.L-1、氯化铵0.6 g.L-1、磷酸二氢钾0.9 g.L-1、硫酸镁0.5 g.L-1、酵母膏1.5 g.L-1,细菌在光照3000 lx、温度(32±2)℃条件下,培养3 d即可达到生长峰值,细菌总数从1.63×109cfu.mL-1提高到3.68×109cfu.mL-1。
The liquid medium for Rhodospirillum branch of photosynthetic bacterium is composed of CH3 COONa,NH4 Cl,MgSO4,KH2 PO4 and peptone.L16(4S)Orthogonal design experiment was applied to optimize the culture medium,taking above 5 components as influencing factors on photosynthetic bacterium growth.According to the results,when the culture medium containing CH3COONa 3.3 g·L^-1,NH4Cl 0.6 g·L^-1,KH2PO40.9 g·L^-1,MgSO40.5g·L^-1 and peptone 1.5 g·L^-1,after 3 days growth under illumination of 3000 lx and temperature of(32±2)℃,the photosynthetic bacterium growth could reach the peak value.The total number of bacterium increased from initial 1.63×109 cfu·mL^-1 to 3.68×10^9 du·mL^-1.The optimized culture medium could effectively improve photosynthetic bacterium growth.
作者
钟为章
罗一菁
张忠智
鲁莽
ZHONG Wei-zhang;LUO Yi-jing;ZHANG Zhong-zhi;LU Mang(Faculty of Chemical Science and Engineering,China University of Petroleum,Beijing 102249,China)
出处
《化学与生物工程》
CAS
2009年第7期67-69,共3页
Chemistry & Bioengineering
基金
中国石油天然气股份有限公司科技风险创新研究项目(20080828)
关键词
红螺菌科
光合细菌
平板菌落计数法
正交实验设计
液体培养基
优化
Rhodospirillum branch
photosynthetic bacterium
plate count for bacterial colonies
orthogonal design
liquid medium
optimization
