摘要
从东北林业大学林场采集黄檗Phellodendron amurense根系及根围土壤,采用Nested-PCR技术扩增黄檗菌根及根围土壤AM真菌18SrDNA NS31/Glol区域,利用该产物进行DGGE分析,并结合DNA测序、系统发育分析及DGGE图谱分析技术对黄檗AM真菌菌群组成进行分析。结果表明:Nested-PCR技术具有较高的灵敏性,可有效地从微量DNA中扩增出约230bp的目的片段;黄檗根系及根围土壤具有不同的DGGE指纹图谱特征,DGGE带谱在条带的数量、亮度、优势度等方面均存在较大差异,全部序列可分为3类菌群,即球囊霉属Glomus、盾孢囊霉属Scutellospora及植物病原菌黄杨亚赤壳Hyponectria buxi,其中Glomus sp.(EF177624)和Glomus sp.(DQ085205)分别为黄檗根系和根围土壤样品中最具优势的AM真菌。
The rhizospheric soil and root samples of Phellodendron amurense were collected in the Logging Station of Northeast Forestry University. Nested-PCR was conducted to specifically amplify NS31/Glol domain sequences of the small-subunit （18S） rDNA from the AM fungi in the root and rhizospheric soil samples of P. amurense, respectively. The Nested-PCR products were applied to denaturing gradient gel electrophoresis （DGGE） analysis. Then, the community composition of the AM fungi was analyzed by DGGE, sequencing, and phylogenetic analysis. The result indicated that the targeted product （230bp） was successfully amplified from trace DNA by Nested-PCR. There were differences in DGGE profiles such as band numbers, densities and dominance between the rhizospheric soil and root samples. All sequences were divided into three groups, viz. Glomus, Scutellospora and Hyponectria buxi. Glomus sp. （EF177624） and Glomus sp. （DQ085205） were the prevalent AM fungi in the root and rhizospheric soil, respectively.
出处
《菌物学报》
CAS
CSCD
北大核心
2009年第4期512-520,共9页
Mycosystema
基金
黑龙江省博士后基金(No.LBH-Z05013)
黑龙江大学博士启动基金
