摘要
背景:大量研究认为降钙素基因相关肽具有刺激成骨、增加骨量,促进神经再生等诸多功能,然而不同制动后降钙素基因相关肽的变化规律及对骨质疏松形成过程中的作用和机制至今仍不十分清楚。目的:观察失神经固定后神经肽物质对大鼠骨密度的影响及其可能机制。设计、时间及地点:随机对照动物实验,于2006-09/11在湘南学院附属医院完成。材料:10周龄SD雄性大鼠96只,体质量220～250g,用于制备失神经支配模型。方法:96只SD大鼠按数字表法随机分为3批,每批4组,每组8只。失神经支配组:大鼠麻醉后,先于俯卧位行两侧后肢股外侧切口,于股骨转子水平切断双侧坐骨神经;再置大鼠于仰卧位,行两侧股正中纵切口,于腹股沟韧带水平切断大鼠两侧股神经,远端游离5mm,缝合切口。固定组:大鼠固定与失神经支配手术同时进行,麻醉后应用管型石膏分别固定1,10,30,60d。对照组行假手术,即在造模过程中仅暴露神经,然后缝合伤口。主要观察指标:实验期间大鼠的一般情况;造模后1,10,30,60d各组大鼠胫骨骨密度和降钙素基因相关肽的变化及降钙素基因相关肽水平与骨密度水平相关性。结果:96只SD大鼠均进入结果分析。①大鼠失神经造模后两后肢无主动屈伸功能,前进时以臀部肌群帮助行走,后退时靠腰腹部肌群收缩代偿,活动量显著减少。固定组大鼠精神好,活动时后肢拖地。对照组大鼠无明显异常。②造模后10,30,60d失神经支配组大鼠胫骨降钙素基因相关肽的表达低于对照组(P<0.05～0.01);而固定组仅造模后30d时与对照组之间存在显著性差异(P<0.05)。造模后10,60d固定组高于失神经支配组(P<0.05～0.01)。③与对照组相比,失神经支配组、固定组造模后30,60d骨密度降低(P<0.05～0.01);失神经支配组和固定组造模30d时骨密度开始下降,60d后下降明显,组内比较差异有显著性意义(P<0.05～0.01)。④失神经后降钙素基因相关肽与骨密度水平变化高度相关(P<0.05);而固定后降钙素基因相关肽与骨密度水平变化相关程度不高。结论:神经的完整性是降钙素基因相关肽水平维持正常的关键,动态观察降钙素基因相关肽水平变化,可在一定程度上预测失神经性骨质疏松形成、发展。
BACKGROUND： It is believed that calcitonin-gene-related peptide （CGRP） can stimulate osteoblast proliferation, increase bone mass, and promote nerve regeneration. However, the change rule, effect and mechanism of CGRP on osteoperosis formation after different immobilization remain unclear. OBJECTIVE： To observe the effect, as well as possible mechanism of neuropeptide on bone mineral density （BMD） after denervation and immobilization. DESIGN, TIME AND SETTING： The Randomized controlled animal experiment was completed in the Affiliated Hospital of Xiangnan University from September to November 2006. MATERIALS： Ninety-six male Sprague Dawley rats, weighing 220-225 g, were prepared denervated models. METHODS： According to random number table, 96 rats were divided into 4 batches, 3 groups in each batch, with 8 rats in each group. The denervated group： after anesthetized, femoral incision was made in the prone position on both sides of hind lateral, and bilateral sciatic nerve at the level of femur was cut off. Next, rats were turned to the supine position, longitudinal incision was made in the median of both sides, femoral nerve on both sides of rats at the level of inguinal ligament were cut of, dissected distally 5 mm and then sutured incision. Immobilized group： rats were performed fixation and denervation surgery simultaneously, then immobilized 1, 10, 30, and 60 days with tude plaster after anesthetization. In the control group, only sham operation was performed. MAIN OUTCOME MEASURES： Ordinary circumstance of rat during experiment; the content change of CGRP and BMD as well as relevance between CGRP and BMD in 1, 10, 30, 60d after the model preparation. RESULTS： All rats were included in the final analysis ①After denervation, there was no initiative expanding or contracting activity with obvious decreased activity amount. Rats in the immobilized group had good spirits, with plucked hind limbs during movement. There was no obviously abnormal in the control group. ② Compared with the control group, the expression of CGRP was lower in the denervated group at days 1, 10, 30, and 60 after model preparation. （P 〈 0.05-0.01 ）, which had statistical difference between the immobilized group and the control group in 30 days after immobilization （P 〈 0.05）. The expression of CGRP was higher in the immobilized group than the denervated group at days 10 and 60 after model preparation （P 〈 0.05-0.01 ）. ③Compared with the control group, BMD content in the immobilized group and the denervated group was decreased at days 30 and 60 after model preparation （P 〈 0.05-0.01 ）, which began to decrease from 30 days, and obvious decreased at 60 days in the immobilized group and the denervated group, there was significant difference betweenintra-group comparison （P 〈 0.05 0.01）. ④ CGRP was highly related to BMD after denervation （P 〈 0.05）. However, the correlative degree was not so high after immobilization. CONCLUSION： The nerve integrity is a key for normal CGRP expression. Dynamic observation of changes of the CGRP content can forecast the formation and development of osteoporosis in a certain extent.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第20期3933-3937,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
湖南省教育厅资助课题(04C170)~~
