摘要
目的用基因芯片技术研究川芎嗪对实验性结肠炎小鼠基因表达谱的影响。方法健康昆明小鼠30只，均分为对照组、0．9％氯化钠组及川芎嗪组。除对照组外，其余小鼠均以恶唑酮灌肠造模。提取0．9％氯化钠组及川芎嗪组结肠组织mRNA，制备cDNA探针，分别用Cy3和Cy5两种荧光染料标记，与基因表达谱芯片进行杂交，经扫描、分析，得出药物作用后表达有差异的基因，并应用荧光定量RT—PCR技术对其中2个表达差异明显的基因（白细胞介素10和白细胞介素4）进行验证。结果川芎嗪组差异表达基因432个，占芯片基因总数的2．86％。其中表达上调的基因307个，表达下调的基因125个，其中部分基因已知其功能。结论川芎嗪通过抑制肠道炎性反应、抗粘附分子及改善免疫功能等多种作用途径为治疗溃疡性结肠炎提供了理论上的可能，但更为精细的治疗靶点还有待进一步探索。
Objective To study the impact of tetramethylpyrazine on genes expression in colitis mice by means of cDNA microarray. Methods Thirty mice were divided into normal control group, 0.9 % NaCl solusion group and tetramethylpyrazine group with 10 in each group. Except the mice in normal group, all mice were treated with oxazolone enema, mRNA was extracted from colons of 0.9 % NaCl solusion group and tetramethylpyrazine group. The cDNA probes were prepared and labeled with dyes Cy3 or CyS. Then the fluorescent probe was hybridized with cDNA microarray and scanned for fluorescent intensity. The differently expressed genes were identified by analysis of gene expression profile. Two differently expressed genes （interleukin-4 and -10） were further validated by fluorogenic quantitative RT-PCR. Results There were 432 （2.86%） differently expressed genes in tetramethylpyrazine group. Of which, 307 genes were up regulated and 125 genes were down- regulated. The function of some genes were well known. Conclusions Tetramethylpyrazine, which can depress inflammatory reaction, anti-adhesive molecule and improve immune systems, is a potential therapy for ulcerative colitis although more detailed curing targes of such medicine need to he further study.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2009年第6期403-406,共4页
Chinese Journal of Digestion
关键词
结肠炎
溃疡性
寡核苷酸序列分析
小鼠
Colitis, ulcerative
Oligonucleotide array sequence analysis
Mice
