摘要
目的:评价次氯酸钠(NaOCl)和氯亚明溶液在相同有效氯浓度下的细胞毒性。方法:按有效氯浓度为0.5000%、0.2500%、0.2000%、0.1500%、0.1250%、0.0625%分别配制NaOCl溶液和氯亚明溶液,使其与L929细胞接触2h、30min、10min,用MTT法检测各组的细胞存活率。结果:在所设定的浓度范围内,除高低两端组(0.5000%-2h及0.0625%-30min)外,细胞在其余各组NaOCl溶液中的存活率均大于氯亚明,差异有统计学意义(P<0.05)。结论:在0.2500%以下的有效氯浓度范围,NaOCl溶液的细胞毒性小于氯亚明溶液。
Objective：To evaluate the cytotoxicity of sodium hypoehlorite（NaOC1） and chloramine-T at a same available chlorine concentration. Methods： L929 fibroblasts were used to test the cytotoxicity of NaOCI and chloramine-T at a same available chlorine concentration （0.500 0%, 0.250 0% ,0.200 0%, 0. 150 0%, 0. 125 0%, 0.062 5% ）. Cell viability after 2 h, 30 min or 10 rain exposure were measured by MTT assay. Results： Except the 0. 500 0% -2 h group and 0. 062 5% - 30 min group, cell viability of NaOC1 groups were higher than chloramine-T group（P 〈 0.05 ）. Conclusion： NaOCl solution has less cytotoxicity than chloramine-T at a same available chlorine concentration lower than 0. 250 0%.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2009年第4期572-575,共4页
Journal of Practical Stomatology
基金
卫生部部属(管)医院临床学科重点项目(卫规财函[2007]353号
编号46)
关键词
根管冲洗剂
次氯酸钠
细胞毒性
Root canal irrigants
Sodium hypochlorite
Cytotoxicity
