摘要
从灰玫瑰青霉Penicillium griseoroseum发酵液中提取到1种具有抗菌活性的物质AF1,对其性质进行初步研究。发酵液经纱布过滤,离心后经5ku超滤膜超滤,经Sephadex LH20和HPLC分离纯化得到纯净物质AF1,并对其生物学活性进行研究。结果证明:AF1对β-内酰胺酶和酸碱稳定,对热有较好的耐受性,不是青霉素类抗菌物质;相对分子质量约为1465,结构中含有糖和氨基特征,对金黄色葡萄球菌的MIC为80μg/mL,对抗药金黄色葡萄球菌有效且不容易诱导细菌抗药性产生。
An antimierobial factor termed AF1 was isolated from culture broth of Penicillium griseoraseum. The filtered fermentation broth underwent eentrifugation and ultrafihration （membrane cutoff 5 kD）. Then it was purified using Sephadex LH-20 and high-perfomumce liquid chromatography （HPLC）. The molecular weight of AF1 was 1465 Da. AF1 was stable to β-laetamase, hydroxylamine, acid, alkali and heat. Together, these data indicate that AF1 is a novel antibacterial factor which is different from penicillin. The molecular structure of AF1 shows characteristics of saceharide and amid. MIC of AF1 versus Staphylococcus aureus is 80 μg/ml, and AF1 is effective to drug-resistant S. aureus. It is difficult to induce bacterial resistant to AF1.
出处
《山东大学学报（理学版）》
CAS
CSCD
北大核心
2009年第7期1-4,共4页
Journal of Shandong University(Natural Science)
基金
国家自然科学基金资助项目(30600026)
山东省优秀中青年科学家科研奖励基金资助项目(2007BS03002)
关键词
青霉
抗菌
分离纯化
生物活性
Penicillium
antibiotic
isolation
characterization
