摘要
OsHSP90是水稻中一类可以在不同逆境条件下被诱导的基因。利用酵母双杂交系统,在水稻低温和干旱cDNA文库中筛选出1个与OsHSP90互作的未知基因,命名为OsHSPBP。序列分析表明,该基因全长1321 bp,编码一个具有244个氨基酸残基的多肽,推测分子量为25.5 kD。在OsHSPBP蛋白的97-132位氨基酸残基间存在tify功能域,185-211位氨基酸残基构成CCT_2基序,与其他植物中的同源蛋白相似性介于30.66%-70.40%。经分析,该基因启动子区域存在6种不同的逆境反应顺式作用元件。RT-PCR半定量结果表明,OsHSP90与OsHSPBP在逆境胁迫下,均能被不同程度地诱导并呈现相似的表达趋势,说明两者可能与水稻逆境胁迫的应答和信号传递有关。
OsHSP90 is an abiotic stress-inducible gene in rice. From the chilling and drought-inducible cDNA library of rice, a novel gene, OsHSPBP, which interacted with OsHSPgO was identified by using yeast two-hybrid system. The 1321 bp full length cDNA of OsHSPBP was cloned. Its open reading frame （ORF） predicts a 244-amino acid（aa） polypeptide with calculated molecular weight of 25.5 kD. A tify motif and a CCT_2 motif were found at the aa 97-132 and aa 185--211, respectively. Sequence similarity analysis indicated that the OsHSPBP had 30.66 %- 70.40% identities with the tify family proteins from other plants. Among the putative promoter region, six factors matched to cis-elements related to the abiotic stress response were identified using the PlantCARE software. Semi-quantitative RT-PCR showed that the OsHSP90 and OsHSPBP might he detected in the similar degree under different stress-treatments, suggesting that OsHSP90 and OsHSPBP could be involved in the signal transmission and response to the abiotic stress in rice.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2009年第4期342-348,共7页
Chinese Journal of Rice Science
基金
教育部长江学者和创新团队发展计划资助项目(IRT0453)
国家科技支撑计划资助项目(2007BAD81B00)
关键词
水稻
逆境胁迫
诱导基因
基因表达
氨基酸序列
酵母双杂交系统
rice
abiotic stressl inducible gene
gene expressionl amino acid sequencel yeast two-hybrid system
