摘要
根据GenBank中公布的普通牛Hepcidin基因序列(登录号为XM-589792.3)设计1对特异性引物。采用RT-PCR技术从牦牛肝脏组织总RNA中扩增出Hepcidin基因的编码序列并进行测序分析,同时构建Hepcidin蛋白物种进化树。结果显示,经克隆获得牦牛Hepcidin基因322bp的cDNA序列(GenBank登录号为EU863791),含289bp的开放阅读框,编码82个氨基酸,包括22个氨基酸的信号肽;预测Hepcidin蛋白分子质量和等电点分别为8.88ku和9.24;与已知普通牛Hep-cidin序列的同源性达99%,不同物种Hepcidin蛋白具有高度的保守性,Hepcidin蛋白物种进化树符合物种进化规律。本研究为Hepcidin基因cDNA全长克隆及基因功能研究奠定了重要基础。
A pair of primers was designed and synthesized according to the bovine Hepcidin gene sequence in GenBank. Coding sequence of Hepcidin gene was amplified by RT PCR technology from total RNA of yak＇s live, the sequence of Hepcidin was analysed and phylogenetic tree of Hepcidin protein was constructed. The results showed that a 322 bp eDNA sequence of Hepcidin gene was cloned, including 289 bp open reading frames, encoding 82 amino acids which contained 22 amino acids coded signal peptide. Forecast for protein molecular weight and isoelectric points, was 8. 88 ku and 9.24 respectively. This gene region had high conservation in the bovine species, compared with the ordinary cow, the homology of Helcidin sequence was up to 99 percent. The phylogenetic tree conformed to the laws of species evolution. The research laid an important foundation on the full-length cDNA gene cloning and study.
出处
《中国畜牧兽医》
CAS
北大核心
2009年第7期88-92,共5页
China Animal Husbandry & Veterinary Medicine
基金
中央级公益性科研院所基本科研业务费专项资金项目(BRF070105)
西北民族大学研究生创新项目(ycx07044)
