摘要
目的探讨噬菌体生物扩增法技术检测不同标本对结核病临床诊断的应用价值。方法采用噬菌体生物扩增法技术对86例活动性肺结核患者痰标本、73例结核性胸膜炎患者胸水、30例肺结核患者肺泡灌洗液、7例结核性脑膜炎患者脑脊液,共计196例结核标本进行检测;对非结核病患者痰标本10例、胸水5例、灌洗液5例,共计20例标本进行检测。每例标本同时进行涂片抗酸染色、罗氏培养。结果86例痰标本、73例胸水、30例肺泡灌洗液、7例脑脊液噬菌体生物扩增法检测阳性率分别为53.49%(46/86)、49.32%(36/73)、60.00%(18/30)、0(0/7),涂片抗酸染色阳性率分别为24.42%(21/86)、2.74%(2/73)、20.00%(6/30)、0(0/7),罗氏培养分别为44.19%(38/86)、4.11%(3/73)、36.67%(11/30)、0(0/7)。20例非结核患者标本,噬菌体生物扩增法、涂片抗酸染色、罗氏培养都未检出阳性。结论噬菌体生物扩增法检测结核病标本的阳性率高于涂片和罗氏培养,该方法敏感性高、特异性强、操作简便,对结核病临床诊断有较高的应用价值。
Objective To evaluate the clinical value of phage amplified biologically （phaB） assay on the detection samples for the diagnosis of mycobacteria tuberculosis. Methods The clinicai samples of mycobacteria tuberculosis including 86 cases sputum of active pulmonary tuberculosis, 73 cases pieurai fluid of tuberculous pleurisy,30 cases bronchial fluid of tuberculosis, 7 cases cerebrospinal fluid of tuberculous meningitis and 20 clinical samples （10 cases sputum,5 cases pieurai fluid and 5 cases bronchial fluid）of non-mycobacteria tuberculosis were detectde by PhaB assay. At the same time each cases were smear and culture. Results The positive rate of PhaB assay in 86 sputum was 53. 49% （46/86）, 73 pleural fluid was 49. 32% （36/73）, 30 bronchial fluid was 60.00%（18/30）, 7cerbrospinal fluid was 0（0/7）. The positive rate of smear and culture were 24.42%（21/86） and 44. 19% （38/86）, 2. 74% （2/73） and 4. 11% （3/73）, 20. 00% （6/30） and 36.67 % （11/30）, 0 （0/7） and 0（0/7）. the positive rate of PhaB assay, smear and culture in 20 clinical samples of non-mycobacteria tuberculosis was 0. Conclusions The positive rate of PhaB assay is higher than smear and culture. This method, which is simple, high sensitive, high specificity. PhaB assy is an efftective clinical method for mycobacteria tuberculosis.
出处
《实用临床医学（江西）》
CAS
2009年第6期11-13,共3页
Practical Clinical Medicine
