摘要
目的研究125I粒子组织间植入对乳腺癌生长的影响及其作用机理。方法采用雌性BLAB/c-nu/nu裸小鼠建立人类乳腺癌MCF-7细胞株的裸鼠皮下移植瘤模型,将荷瘤鼠分为2组,对照组(n=40):植入空载粒子,无125I放射性元素(0 Bq);实验组(n=40):瘤体长径达8~10 mm时植入125I粒子(1.48×107Bq)。植入后每隔3 d观测肿瘤大小,绘制肿瘤生长曲线,计算抑瘤率;以对照组瘤体平均长径达15~20 mm时为处死动物的时间,2组各处死30只,取完整肿瘤组织称重,并行常规病理组织学检查;剩余裸鼠观察90 d内的自然生存状态。结果在90d内,对照组荷瘤鼠的平均存活时间为56.2 d,实验组的平均存活时间为74.8 d,2组生存时间比较差异有统计学意义(P<0.05)。从2组的肿瘤生长曲线看,对照组的肿瘤生长曲线高抬,而实验组的肿瘤生长曲线一直低平。植入125I粒子后,实验组的肿瘤生长明显减慢,抑瘤率为55.21%;对照组平均瘤重为(3.26±0.39)g,实验组平均瘤重为(1.46±0.17)g,2组比较差异有统计学意义(■=22.896 2,P<0.05)。光镜下见,实验组较对照组癌细胞数量减少,核碎片增多,癌巢结构不明显。结论125I粒子组织间植入可有效抑制乳腺肿瘤组织生长,可能与125I粒子直接辐射杀伤肿瘤细胞或诱导其凋亡及抑制肿瘤血管的新生有关。
Objective To investigate the effect of iodine-125 on inhibiting breast cancer growth and to explore its possible mechanism. Methods The animal model of the MCF-7 tumor was established firstly through injection of the cells into nude mice, The animals were divided into two groups before the implantation of the iodine-125 granule into the tumor mass： control group （n= 40, to implant no-load seeds, non-iodine-125 radioelement） and experimental group [n=40, to implant iodine 125 seeds （1. 48×10^7 Bq） when the length of tumor was 8-10 mm]. The width and length of tumor, in order to calculate the volume, were measured every three days to observe tumor growth curve and to calculate the rate of the tumor inhibition. When the length of tumor was 15-20 mm in the control group, 30 nude mice were killed in every group to detect the weight of tumor and histopathological changes. Other ten nude mice of each group were remained to be observed the national life span and survival rate for 90 days. Results Within 90 days, the average survival time in control group and experimental group were significantly different （56.2 d vs. 74.8 d, P〈0.05）. In control group the growth curve was continuously elevated, while experimental group showed a low flat curve. With iodine-125 treatment, the tumor growth decreased in experimental group with tumor inhibition rate 55.21%. The average tumor weight in control group and experimental group was （3. 26±0.39） gand （1.46±0.17） g （t＇=22.896 2, P〈0.05）. As compared with control group, under light microscope, the number of cancer cells was less, nuclear debris increased, and cancer structure was not obvious in experimental
出处
《中国普外基础与临床杂志》
CAS
2009年第7期510-513,共4页
Chinese Journal of Bases and Clinics In General Surgery
基金
国家自然科学基金资助项目(项目编号:30660183)
云南省教育厅科学研究基金资助项目(项目编号:06y091c)~~
关键词
乳腺癌
125I粒子
植入
放疗
Breast cancer
Iodine-125 seed
Implantation
Brachytherapy
