摘要
对引起双孢蘑菇(Agaricus bisporus)褐腐病的病原菌进行分离和分子鉴定。分离培养褐腐病病原菌,采用CTAB法抽提其基因组总DNA,利用真菌通用引物ITS1和ITS4扩增菌株rDNA ITS区序列,扩增产物纯化后克隆转化至大肠杆菌,挑选克隆成功的菌株进行测序。测序结果在GenBank中进行同源性搜索,并下载部分具有代表性种的ITS序列,利用软件MEGA4构建分子系统发育树,通过序列分析,鉴定出引起双孢蘑菇褐腐病的病原菌为Mycogoneperniciosa。
Strain CSUFT0821, a pathogen causing brown rot of Agaricus bisporus, has been identified by determining the sequence of a ribosomal DNA internal transcribed spacer （ITS） and comparing with corresponding sequences deposited in GenBank. Homology was highest （99%） with an ITS sequence from Mycogone perniciosa （EU380317）. A phylogenetic tree constructedon the basis of ITS sequences revealed.very close proximity between EU380317 and the ITS sequence from strain CSUFT0821, and confirmed that the latter was M. perniciosa.
出处
《食用菌学报》
北大核心
2009年第2期74-76,共3页
Acta Edulis Fungi
基金
国家林业局"948"项目(编号:2004-4-28)的部分研究内容
关键词
双孢蘑菇
褐腐病
转录间隔区
分子鉴定
Agaricus bisporus
brown rot
internal transcribed spacers （ITS）
molecular identification
