摘要
目的:比较酶联免疫吸附试验(ELISA)和逆转录聚合酶链反应(RT-PCR)在检测丙型肝炎病毒感染者血清中抗-HCV与HCV-RNA结果是否存在差异。方法:采用ELISA法和RT-PCR法对109例丙型肝炎患者和41例健康体检者的血清进行抗-HCV与HCV-RNA检测。结果:150份标本中抗-HCV与HCV-RNA均阳性者81份,占54.00%(81/150),抗-HCV阴性HCV-RNA阳性12份,占8%(12/150),抗-HCV阳性HCV-RNA阴性18份,占12%(18/150),两者均阴性的39例,占26%(39/150)。两种检测方法总符合率为80%,RT-PCR法特异度98%、敏感度92%。两者检测经统计学处理差别无统计学意义(P>0.05)。结论:抗-HCV抗体检测与荧光定量RT-PCR检测在丙型肝炎诊断中无显著差异,但二者联合检测可提高检出率。
Objective： To compare the ELISA （ELISA） with reverse transcription polymerase reaction （RT- PCR） in the hepatitis C virus infection in the anti -HCV and HCV -RNA with the results of the difference, nethods：ELISA and RT- PCR method to 109 cases of hepatitis C patients and 41 cases of physical examination of the anti - HCV detection and HCV - RNA. Results ： 150 specimens anti - HCV and HCV - RNA both positive 81, accounting for 54.00% （81/150）, anti - HCV positive negative HCV - RNA 12, accounting for 8% （ 12/150）, anti - HCV positive HCV - RNA negative 18 Copies, accounting for 12% （ 18/150）, both of which were negative in 39 cases, accounting for 26% （39/150）. Two line detection rate of 80%, differential 98% and sensitive 92% Of RT - PCR. Detection of the two statistical difference was not significant P 〉 0. 05. Conclusion： Of anti - HCV body detection and fluorescence quantitative RT - PCR test in the diagnosis of hepatitis C is not significantly different, but the two combined detection can improve the detection rate.
出处
《中国卫生检验杂志》
CAS
2009年第7期1562-1563,共2页
Chinese Journal of Health Laboratory Technology
