摘要
以细辛叶片为外植体,研究了其组织培养技术。结果表明：愈伤组织继代1次,可增殖10-15倍,每块愈伤组织经过再分化能长出5-8株试管苗,反复继代3-4次,每个叶片可产出约500株试管苗。
Used the leaf of Asarum siboldii as explants, the tissue culture technique was studied. The results showed that the the callus could proliferate 10 - 15 times after subcuhuring for one time. 5 - 8 plantlets grew out when the callus dedifferentiated. When the callus was subcuhured for 3 -4 times, about 500 plantlets could be obtained.
出处
《河北农业科学》
2009年第7期39-39,117,共2页
Journal of Hebei Agricultural Sciences
关键词
组织培养
细辛
试管苗
Tissue culture
Asarum siboldii
Plantlet
