摘要
目的:对红金耳环中马兜铃酸A进行定量分析。方法:采用高效液相色谱(HPLC)法测定马兜铃酸A的含量,色谱柱:Kro-masi C18(250 mm×4.6 mm,5μm),流动相甲醇-水-冰醋酸(65∶34∶1),流速1.0 ml.min-1,检测波长310 nm,柱温25℃。结果:马兜铃酸A在0.16～0.80μg范围内呈良好的线性关系,r为0.9995,平均加样回收率为98.97%,RSD为2.10%。红金耳环中马兜铃酸A的含量为0.0012%。结论:该方法简便,重现性好,可用于测定红金耳环中马兜铃酸A的含量。
Objective：To analyse aristolochic acid A in Asarum petelotii O.C. Schmidt in order to guide use in clinic. Methods：The content of aristolochinc acid A in Asarum petelotii O.C. Schmidt was determined by HPLC on Kromasi C18（250 mm×4.6 mm,5 μm） with the mobile phase consisted of methanol-water-glacial acetic acid （65：34：1）. The flow rate was 1.0 ml. min^-1.The detection wavelength was 310 nm. Column temperature was set at 25 ℃.Results：The method of HPLC had a good linearity relationship in the range of 0.16-0.80 μg （r=0.999 5）.The average recovery was 98.97% （RSD=2.10%）. Conclusion：HPLC used in content determination of aristolochinc acid A is precise and reproducible,which can be used for precisely analysing the quantity of aristolochic acid A in Asarum petelotii O.C. Schmidt.
出处
《现代医药卫生》
2009年第15期2245-2246,共2页
Journal of Modern Medicine & Health
