摘要
[目的]了解现有新城疫疫苗对近年流行新城疫病毒(NDV)毒株的保护效果,从而为新城疫疫苗的合理应用和新城疫的防制提供指导。[方法]用1个或0.01个使用剂量的鸡新城疫弱毒活疫苗A、B、C、D、E经滴鼻、点眼、口服途径分别免疫6周龄SPF鸡。免疫14 d后,测定血清中HI抗体效价,同时分别用NDVF48E9标准强毒株和野外分离强毒株NDV-2007-HB、NDV-2008-YB攻毒。连续观察攻毒鸡的表现并统计发病和死亡情况。[结果]5种疫苗免疫14 d后,均可诱导鸡只产生保护水平的抗体。1个剂量或0.01个剂量的现有疫苗对NDVF48E9株的攻毒保护率均为100%,对NDV-2007-HB的攻毒保护率均大于90%;0.01个剂量的疫苗A、B、C可提供对NDV-2008-YB的攻毒保护率均大于90%。[结论]实验室条件下,现有新城疫疫苗对分离到的2株NDV毒株具有较好的保护效果,能预防鸡新城疫的发生。
[Objective] To investigate the protective efficacy of currently available Newcastle disease vaccines against currently prevalent strains and thus provide guidance for the application of vaccines and the prevention and control of Newcastle disease. [ Method] The 6-weekold SPF chickens were respectively inoculated with five live attenuated Newcastle disease vaccines （ A, B, C, D, E） at the dose of 1-fold or 0.01-fold usage via intranasal, intraocular or oral route. After 14 d post immunization, the titers of HI antibody were detected. And all the chickens were challenged by Newcastle disease virus （NDV） F48E9 standard strain or the isolated wild strains, NDV-2007-HB and NDV- 2008-YB. The clinical symptoms of chickens were continuously observed, and the morbidity and mortality were determined. [ Result] After 14 d post immunization, antibodies were induced at a protective level in chickens immunized with the five vaccines. As shown by the animal experiment, the five Vaccines at the dose of 1-fold or 0.01-fold usage protected all vaccinated chickens from the death caused by NDV F48E9 strain, and more than 90% of vaccinated chickens from the death caused by NDV-2007-HB strain, while the vaccines, A, B, and C, at the dose of 0.01-fold usage protected more than 90% of vaccinated chickens from the death caused by NDV-2008-YB strain. [ Conclusion ] Under laboratory conditions, the currently available Newcastle disease vaccines have better protective efficacy against the two currently prevalent NDV strains and prevent the occurrence of Newcastle disease.
出处
《安徽农业科学》
CAS
北大核心
2009年第23期11021-11022,11028,共3页
Journal of Anhui Agricultural Sciences
关键词
新城疫
疫苗
流行毒株
保护
Newcastle disease
Vaccine
Prevalent strains
Protection
