摘要
以大肠杆菌为代表材料,用扫描电镜观察比较用PBS和蒸馏水清洗再制样的图象效果,对比固定与不固定,用自然晾干法、烘干法和用梯度酒精脱水再冷冻干燥法处理的大肠杆菌图像。提出用蒸馏水替代PBS清洗微生物样品以避免干燥后被固定液和PBS残留物覆盖的方法,明确具有细胞壁的细菌也需固定,对单细胞生物用梯度酒精脱水再冷冻干燥要比自然晾干和烘干脱水更彻底。
With Escherichia coli as representative materials, the image effects of Escherichia coli after being bathed with BIaS and distilled-water were observed by using SEM. The conformations of the fixed and non-fixed samples were examined. The aired, warm-incubated and freezingly-evaporated bacilli were compared. The results indicated that water bathed sample was mare clear than that with PBS, for water sweeped away the residues of fixing solution and PBS which left on and covered the surface of samples when dried. Although the cell wall can maintain the conformation, the bacterium can secrete out its inner substances if do not fix. Dehydrating with the series of concentrate inereasing aehol and then freezingly evaporating were better ways than airing or warm-incubating for drynes.
出处
《安徽农业科学》
CAS
北大核心
2009年第23期10886-10888,共3页
Journal of Anhui Agricultural Sciences
基金
浙江省测试基金(04165)
南京农业大学生命科学实验中心开放课题基金(680810150)
关键词
扫描电镜
样品制作
微生物
大肠杆菌
Scanning electric microscopy
Sample preparing
Microorganism
Escherichia coil
