摘要
本文用１０倍稀释法确定了河流弧菌Ⅱ在Ｃｕ，Ｚｎ，Ｓｅ，Ｃｄ离子中的半致死浓度是３．５６×１０－２、４．４６×１０－３、３．６９、２．７８×１０－５μｇ／ｍｌ。细菌吸收了这几种离子后其超微结构发生了明显变化。这些离子能诱导细菌内的核糖体增多，ＤＮＡ纤丝凝聚成棍棒或块状。以Ｃｕ、Ｚｎ离子诱导ＤＮＡ纤丝聚集较为明显，类核区扩大或出现透明区，而Ｓｅ、Ｃｄ离子处理的菌核区不明显。而且４种离子对细胞壁也有不同程度的破坏，Ｚｎ离子使细胞壁粗糙与胞膜分离明显，Ｃｕ、Ｚｎ离子处理的样品细胞质集中在周边，细胞质内出现明显的电子致密颗粒。尤其是４种离子混合作用的细菌，上述现象更为明显。Ｓｅ离子毒性相对较弱，细菌的超微结构没有明显的变化。Ｃｄ离子处理的细菌超微结构变化明显。首先，细胞壁呈泡状外突，最后小泡处破裂。细胞质内出现许多电子密度大的颗粒（可能是聚缩的ＤＮＡ），类核区不明显，但范围比对照正常菌明显扩大，其内的ＤＮＡ纤丝聚缩成短棍状或块状，散布在细胞质中。
This paper reported semilethal concentration of Cu,Zn,Se and Cd ion to Vibrio fluvialis Ⅱby ten times dilution method,it was found to be 356×10-2、446×103、369or278×105μg/ml respectively.After the bacteria absorb Cu,Zn,Se and Cd,their ultrastructures were changed.These ions could induce the increase or bacteria ribosome,the DNA aggregated to stick of cube form.The Cu,Zn ion induced DNA fibril clearer than Se,Cd ion induced.The Cu,Zn ions also induced nucleoid area expanded or transparent area appeared,while the transparent area did not appeared with Se,Cd treated.Four ions could destory their cell wall.Zn ion coarsened cell wall and made cell membrane seperated from the wall.The cytoplasma of Cu and Zn ions treated bacteria concentrated on periphery.If a mixture of Cu,Zn,Se and Cd ions treated the bacteria the phenomenon became clearer.Toxicity of Se ion was weak,bacteria ultrastructure did not change.Cd ion changed bacteria ultrastructure prominently.First,cell wall presented bubble protruding,later bubble bursted out.There were a lot of high density electron particle in bacteria cytoplasm,nucleoid area was not clear.DNA fibril aggregated to stick or cube Which dispersed in cytoplasm.
出处
《水产科学》
CAS
北大核心
1998年第4期3-9,共7页
Fisheries Science
基金
国家农业部资助
大连市科委资助
