摘要
目的:建立一种测定氨磷汀聚乳酸乙醇酸共聚物(PLGA)微球中氨磷汀含量的方法。方法:样品与荧光胺试剂碱性条件下衍生化后,以Agilent HC-C18柱(250mm×4.6mm,5μm)分离,以乙腈-水-10%磷酸溶液(25∶75∶1)为流动相,流速为1mL.min-1,以荧光检测器检测,激发波长为395nm,发射波长为480nm。结果:氨磷汀样品在0.4～4.0mg.L-1范围内与其峰面积有良好的线性关系(r=0.999 9)。低、中、高3个浓度的回收率(n=9)分别为95.83%,94.66%,96.15%;RSD分别为1.6%,1.8%,1.5%。结论:该方法灵敏度高,准确迅速,重复性好,能够满足氨磷汀微球中氨磷汀含量的分析测定。
OBJECTIVE To establish an HPLC method with pre-column derlvatlzation to cletermme amifosnne in amifostine PLGA microsphere. METHODS After pre-column derivatization with fluorescamine at alkaline condition, the derivatives were injected and separated on a Agilent HC-C18 column （250 mm × 4. 6 mm,5 μm）, with acetonitrile-water-10% phosphoric acid （25 ：75：1） as mobile phase. The fluorescence detector was operated at excitation and emission wavelengths of 395 and 480 nm, respectively. The flow rate was 1. 0 mL min^-1. RESULTS Amifostine derivatives showed good linearity （r = 0. 999 9） in the range of 0. 4 - 4. 0 mg · L ^-2. The average recoveries （r = 3） of low, middle and high concentration were 95.83%, 94. 66%, 96. 15 % with RSD of 1.6%, 1.8%, 1.5 % respectively. CONCLUSION The method is simple, rapid, reliable and accurate.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2009年第14期1189-1192,共4页
Chinese Journal of Hospital Pharmacy
基金
西北工业大学研究生创业种子基金(编号:200874)
