摘要
将５６头１３～１８月龄育成牛随机分成７组，每组８头，第１组不添加铜作对照组，第２，３，４组日粮分别添加５，１５和２５ｍｇ·ｋｇ－１硫酸铜，第５，６，７组日粮分别添加５，１５和２５ｍｇ·ｋｇ－１蛋氨酸铜，于试验的０，５，２０和４０ｄ分别采集日粮，牛毛和血液进行铜，血浆铜蓝蛋白和红细胞超氧化物歧化酶活性测试。观察两种铜源的立刻效应（５ｄ）和长远效应（４０ｄ），找出干旱地带奶牛添加不同铜源的最佳地方化标准，并比较两者的生物学利用率。结果表明，育成牛日粮铜为４．６７ｍｇ·ｋｇ－１，牛毛铜为６．４６ｍｇ·ｋｇ－１，全血铜为０．５９８７ｍｇ·ｋｇ－１，均低于ＮＲＣ标准，说明试验牛处于缺铜状态。试验的第５ｄ。红细胞超氧化物歧化酶活性，１５和２５ｍｇ·ｋｇ－１硫酸铜组，５ｍｇ·ｋｇ－１蛋氨酸铜组与本底值差异显著（Ｐ＜００５），１５和２５ｍｇ·ｋｇ－１蛋氨酸铜组与本低值差异极显著（Ｐ＜０．０１）。第２０ｄ蛋氨酸铜各剂量组全血铜测值上升幅度大，但组间差异不显著，与其相应的本底值和对照组值差异也不显著，第４０ｄ测值与第２０ｄ一样。血浆铜蓝蛋白第２０～４０ｄ时除对照组和５ｍｇ·ｋｇ－１硫酸铜组与其相应的本底值差异不显著外，其余各?
おifty six 13～18 months old growing cattle were allotted randomly to seven groups, and assigned to dietary treatments of 1) non-supplemental copper as control, 2) copper sulfate groups of 2,3,and 4 with copper sulfate supplements of 5,15 and 25 mg·kg-1,respectively and 3) copper methionine chelate The dietary,hair and blood samples were obtained for measure in blood copper concentration, ceruloplasmin(CP) and erythrocyte copper-zinc superoxide dismutase (ECu-ZnSOD) activities, which were to assess relative Cu bioavailability, Cu short-term effect(5 days) and longterm effect (20～40 days) from different Cu sources for growing cattle, and also to find out the best local standard for different Cu sources of dairy cow in desert area The results conclude: Cu concentrations in the basic dietary, hair and blood were 467,646 and 05987 mg·kg-1, respectively All of them were lower than NRC (7th Edit) requirement for dairy cattle This means that the experiment cattle were all deficient in blood Cu and the ceruloplasmin with different Cu sources and different dosage were not significantly defferent (P>005) among the six groups on day 5, compared with each base line (od values), the results were also not significant different (P>005), but the values were higher with increased dosage ECu-ZnSOD activity was significantly higher (P<005) on day 5 in cattle fed with 15～25 mg·kg-1 CuSO4 and 5 mg·kg-1 Cu(met)2 than the activity of each base line ECu-ZnSOD activity was highly significance different (P<001) at day 5 in cattle fed with 15,25 mg·kg-1 Cu(met)2 from the activity of each base line Ceruloplasmin and ECu-ZnSOD were significant different (P<005) or highly significant different (P<001) in each group on the days 20～40 except for control group and 5 mg·kg-1 CuSO4 group On the day 40th, ECu-ZnSOD activity maitined stability in Cattle fed with 25 mg·kg-1 Cu(met)2 By further analysing, ceruloplasmin activity was found to be significant different(P<005) between the cattle fed with 15 mg·kg-1 CuSO4 and 5 mg·kg-1 Cu(met)2 on the 40th day ECu-ZnSOD activity had been significant different in the above two groups on the 5th day, the rgre correlaltion coefficient of 15 mg·kg-1 CuSO4 between blood copper and ceruloplasmin was r=089, the bioavailability of CuSO4 was as follows: 1) taking base line of 15 mg·kg-1 CuSO4 as index 100, 9513, 9810 amd 10063 respectively on the 5th, 20th and 40th day The correlation coefficient of 5 mg·kg-1 Cu(met)2 between blood copper and ceruloplasmin was r=057,blood copper and ECu-ZnSOD was r=083, ceruloplasmin and ECu-ZnSOD was r=088 The bioavailability of Cu(met)2 was as follows:2) taking the base line of 5 mg·kg-1 Cu(met)2 as index 100, 10043,10835 and 10894 respectively on the 5th, 20th and 40th day 15 mg·kg-1 CuSO4 and 5 mg·kg-1 Cu(met)2 were required for the production in growing cattle on the desert area
出处
《东北农业大学学报》
CAS
CSCD
1998年第2期122-132,共11页
Journal of Northeast Agricultural University
关键词
乳牛
育成牛
铜源
生物利用率
coppers, growing cattle, desert area, bioavailability
