摘要
用HPLC-ELSD法分离与测定蔗糖酯,考察了蔗糖酯的HPLC-ELSD分析条件。用Hypersil C8色谱柱(250 mm,4.6 mm,5μm),以〔V(甲醇)∶V(四氢呋喃)=90∶10〕-水二元溶剂系统为流动相,梯度洗脱,流动相流速1.0 mL/m in,柱温40℃,对商品蔗糖酯和合成蔗糖酯进行了定性和定量分析。研究结果表明,在该条件下,HPLC-ELSD有效地分离了蔗糖酯产品中的蔗糖、蔗糖单酯、蔗糖二酯、脂肪酸、硬脂酸甲酯和蔗糖多酯,不但可用于蔗糖酯合成中的终点控制,而且可以对精制后的产品进行分析,从而监控产品的质量,也可用于蔗糖酯的结构鉴定,是一种连续、快速、方便、高效的蔗糖酯的定性和定量分析方法。
The commercial and synthesized sucrose esters were analyzed with HPLC -ELSD method. Their separation and determination were also carried out. Samples were analyzed by a hypersil C8 reversed phase column （250 mm ,4. 6 mm, 5μm particle size） using V（methanol） ： V（tetrahydrofuran） = 90：10 and water under gradient conditions as the mobile phase, the flow rate was 1.0 mL,/min and the column temperature was set at 40 ℃. This procedure provided a complete separation and determination of sucrose, sucrose monoester, sucrose diester, fatty acid, methyl stearate and sucrose higher esters in each fraction on a single run. The described method is found to be straight forward, rapid ,efficient and can be readily applied for the analysis of sucrose esters during the processes of synthesis, purification, and structure studies.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2009年第7期633-638,共6页
Fine Chemicals
基金
南通市科技局项目(K2007011和K2008023)
南通大学自然科学基金项目(07Z020
08R08)~~
关键词
蔗糖酯
HPLC-ELSD
分离与测定
定性分析
定量分析
表面活性剂
sucrose esters
HPLC - ELSD
separation and determination
qualitative analysis
quantitative analysis
surfactants
