摘要
以麻疯树花粉处于单核中晚期的花药作为实验材料,探讨了麻疯树花药培养从愈伤组织诱导,愈伤组织分化芽,壮苗,生根的整个过程.其中愈伤组织诱导的最好培养基为 N6+2,4-D 1.0mg/L+KT0.4mg/L+10%蔗糖,避光培养;在愈伤组织分化芽的过程中,TDZ 起到了非常明显的效果,最佳的培养基为 MS+TDZ2.0 mg/L+IAA 2.0mg/L+水解酪蛋白1g/L+3%蔗糖;最合适的壮苗培养基为 MS+GA3 1.0mg/L+6-BA 0.5mg/L+IBA1.0mg/L+3%蔗糖;生根效果最好的是1/2MS+IBA 0.2mg/L+IAA 0.2mg/L+3%蔗糖,生根率40%.
The microspores at late uninucleate stage were used as explants in anther culture of Jatropha curcas L.. The processes include callus induction, callus differentiation, shoots growth, and rooting. The most efficient callus induction medium is N6＋2,4-D 1.0 mg/L＋KT 0.4 mg/L＋10% sucrose and the cultures should be kept in darkness. The best differentiation medium is MS＋TDZ 2.0 mg/L＋IAA 2.0 mg/L＋CH 1g/L＋ 3% sucrose and the TDZ plays an important role during the shoots differentiation. The optimal culture medium for shoots growth is MS＋GA3 1.0 mg/L＋6-BA 0.5 mg/L＋ IBA 1. 0 mg/L＋3% sucrose. The best rooting medium is 1/2 MS＋IBA 0.2 mg/L ＋IAA 0.2 mg/L＋3% sucrose and the rooting rate is 40 %.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第4期1120-1124,共5页
Journal of Sichuan University(Natural Science Edition)
基金
国家"十一五"科技支撑计划课题(2007BAD50B05)
关键词
麻疯树
花药培养
愈伤组织诱导
愈伤组织分化
Jatropha curcas L. , anther culture, callus induction, callus differentiation
