摘要
建立了鱼肉中甲基汞、乙基汞和苯基汞残留量的高效液相色谱串联原子荧光光度计定量测定方法。样品经丙酮脱脂、盐酸消解,二氯甲烷萃取,水封吹氮浓缩等处理,利用高效液相色谱分离,经形态分析预处理装置后,有机汞被氧化为无机汞,原子荧光光度计检测。添加浓度在2~50μg/kg时,甲基汞、乙基汞和苯基汞平均回收率在75.8%~84.2%之间,变异系数在3.5%~7.8%之间。以S/N=3计算,甲基汞、乙基汞和苯基汞方法检出限分别为1、2、4μg/kg。该方法前处理简单、回收率稳定、灵敏、准确,杂质干扰少,适用于鱼肉中甲基汞、乙基汞和苯基汞残留量的分析。
A method for the determination of methyl mercury, ethylmercuric and phenylmercury residues in fish were developed using HPCL-AFS method. Sample was defatted by acetone, digested by HCl, extracted by dichlommethane and then water-enling Ireated with blowing nitrogen, before HPLC-AFS detection. Results showed that, within the ranges of concenlration of 2-50 μg/kg, the recoveries of the three enmpounds ranged from 75.8% to 84.2%, and the eoeificients of variation were between 3.5% and 7.8%. The limits of method for methyl mercury, ethylmereuric and phenylmercury residues were 1 μg/kg, 2 μg/kg and μg/kg, respectively （S/N=3）. This method was simple, sensitive and precise, which could be used for qualification and quantification analysis requirement for methylmercury, ethylmereuric and phenylmecury residues in fish.
出处
《现代食品科技》
EI
CAS
2009年第7期838-840,共3页
Modern Food Science and Technology
基金
广东省科技计划项目(No.2007B080401015
2007A020300008-4)
