摘要
硫醇是生物体中许多蛋白质和小分子的重要组成部分,在细胞的抗氧化系统中具有重要的作用,定量检测硫醇在生物化学和临床化学中具有重要的意义。荧光法由于其具有灵敏度高、能够实现对活体甚至单个细胞的实时可视化示踪的优点,成为目前广泛采用检测细胞内硫醇类物质的一种重要手段。本文引用文献51篇,按荧光探针与巯基作用机理的不同分类,就近年来该领域的研究进展做了比较系统的评述,并展望了此类探针的发展趋势和应用前景。
Thiols, which are components of many proteins and simple molecules, play an important role in the cellular antioxidant defense system. The quantitative determination of thiols is important in biochemistry and clinical chemistry. Fluorescent probes, which have its apparent advantages in sensitivity and, most importantly, in imaging thiols in vivo, even in single living ceils, appear to be particularly attractive. In this review, we classify the fluorescent probes based on their different reaction mechanisms with thiols and summarize the recent progresses of thiols fluorescent probes with fifty-one references. Particularly, the research actuality, the developing trends and prospects in the future were also discussed.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2009年第7期1073-1081,共9页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金重大研究计划(No.90713019)
国家自然科学基金(Nos.20575036,20875057)
国家重点基础研究发展计划(No.2007CB936000)
国家杰出青年基金(No.20725518)
山东省自然科学基金重点项目(No.Z2006B09)
教育部博士点基金(No.20060445002)资助
关键词
硫醇
荧光探针
综述
Thiols, fluorescent probe, review
