摘要
目的观察ＩＦＮγ对培养大鼠肝细胞ＤＮＡ和胶原的影响．方法应用３Ｈ胸腺嘧啶核苷和３Ｈ脯氨酸掺入量及掺入抑制率测定的方法．结果在５ｋＵ／Ｌ～１００ｋＵ／Ｌ浓度范围内的ＩＦＮγ能明显抑制培养大鼠肝细胞ＤＮＡ（１００ｋＵ／Ｌ为６４６％）和胶原（１００ｋＵ／Ｌ为６７４％）合成，抑制程度与浓度和时间呈正相关（Ｐ＜００１）．
AIM To study the effect of interferon γ on DNA synthesis and collagen production of cultured rat hepatocytes. METHODS To assay the incorporation of 3 H thymidine and 3 H proline into cultured hepatocytes. RESULTS Interferon γ in concentrations of 5kU/L - 100ku/L inhibited DNA synthesis (100kU/L,64 6%) and collagen (100kU/L, 67 4%) production of the cells, and the inhibition is time and dose dependent. CONCLUSION The mechanism of interferon γ antifibrogenic effect is by inhibiting hepatocyte activation and collagen production.
关键词
肝硬化
预防
代谢
DNA
生物合成
胶原
liver cirrhosis/prevention & control
liver/metabolism
DNA/biosynthesis
collage/biosynthesis
interferon type Ⅱ/pharmacology
