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Ghrelin及其受体在兔淋巴器官的分布及发育性变化 被引量：1

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摘要 利用免疫组化法研究Ghrelin在3月龄新西兰大白兔免疫器官内的分布定位。结果显示:Ghrelin免疫阳性细胞分布于脾、胸腺和腘淋巴结,以脾中的分布密度为最高。利用RT-PCR方法研究Ghrelin及其受体在1,3,6和12月龄兔免疫器官内的发育性变化。PCR结果显示:Ghren-lin及其受体mRNA在兔脾、胸腺和腘淋巴结均表达,Ghrenlin mRNA在腘淋巴结的表达水平较高,在脾脏和胸腺的表达较低;3种器官内Ghren-lin基因表达的发育变化趋势一致,以1月龄和3月龄表达较高,6月龄和12月龄较低。Ghrenlin受体mRNA的表达以胸腺为最高,在其他2种器官内的表达基本相似;胸腺内Ghrenlin受体基因表达的发育变化趋势与Ghrenlin基因表达的趋势一致,但在其他2种器官内的发育性变化则相反,以12月龄和6月龄较高。这表明Ghrelin及其受体在兔免疫器官内呈发育性变化。
作者 李月 朱向蕾 杨桂红 杨虹 应志豪 姚远 苏娟 雷治海
机构地区 南京农业大学动物医学院
出处 《畜牧与兽医》 北大核心 2009年第7期63-66,共4页 Animal Husbandry & Veterinary Medicine
基金 南京农业大学SRT计划项目(0704A03)
关键词 GHRELIN Ghrelin受体 新西兰大白兔 免疫组化 RT—PCR
分类号 S852 [农业科学—基础兽医学]
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参考文献11

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二级参考文献42

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共引文献36

同被引文献17

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畜牧与兽医
2009年 第7期

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