摘要
对小白菜品种进行游离小孢子培养,得到9株再生植株,其中2株为单倍体,7株为二倍体,二倍体自然加倍率为77.7%。为避免材料间差异,取单倍体和二倍体再生植株各2株,提取单株DNA,分别构建单倍体和二倍体DNA混合池。利用甲基化敏感性限制性内切酶-PCR法,结合SRAP分子标记技术,对2个DNA混合池进行筛选,共筛选768对引物,只有在未酶切的单倍体和二倍体DNA池中SRAP引物扩增无差异,而酶切后有差异的条带,才认为是单倍体和二倍体甲基化差异。试验共获得8对含有多态性的引物,试验结果证明小白菜小孢子培养获得的再生植株的倍性与基因组DNA甲基化有关。
By isolated microspore culture technique from pakchoi （Brassica rapa ssp. chinensis）, 9 regeneration plants including 2 haploids and 7 diploids were obtained. The spontaneously double rate of diploid was up to 77.7 %. Two haploids and 2 diploids were selected to construct their respective DNA pool. Combining Methylation-Sensitive Restriction Endonuclease -PCR method with SRAP marker technique, it gained 8 primer combinations showing polymorphism from 768 pairs of primers. It indicated that the ploidy level of regeneration plants from pakchoi may be related to DNA methylation in plant genomics.
出处
《中国蔬菜》
北大核心
2009年第14期12-16,共5页
China Vegetables
基金
农业部蔬菜遗传与生理重点开放实验室资助项目
