摘要
目的了解新生儿病房医院感染病原菌的分布及耐药性。方法采用法国生物梅里埃公司的BacT/Alert 3D进行血液培养,菌株鉴定采用法国生物梅里埃公司的VITEK60分析仪,药敏试验采用K-B法,纸片扩散确证试验检测超广谱β-内酰胺酶(ESBLs)。结果血培养中76.5%的菌株是凝固酶阴性葡萄球菌,80.0%肺炎克雷伯菌主要分离于咽拭子及痰液标本,产ESBLs大肠埃希菌为57.1%,产ESBLs肺炎克雷伯菌为87.2%。结论医院必须加强医院感染控制,医生应依据药敏结果选择合适抗菌药物。
OBJECTIVE To study the distribution of pathogens of nosocomial infection in neonate ward and analysis of their drug resistance. METHODS Blood was cultured in BacT/Alert3D of Bio-Merieux of France, The clinical isolates were identified by VITEK-60 of Bio-Merieux of France. Using K-B agar diffusion method and confirmed test, the drug resistance of extended spectrum β-lactamases （ESBLs） producing Klebsiella pneumoniae and Escherichia coli were detected. RESULTS The incidence of coagulase-negative staphylococcus was 76. 5% in blood culture, K. pneumoniae was mostly isolated from sputum and throat swab （80.0%）, The incidence of ESBLs was 57.1% in E. coli and 87.2% in K. pneumoniae. CONCLUSIONS We must control nosocomial infection, doctors should choose proper antibiotic and according to analysis of their drug resistance.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第13期1716-1717,1727,共3页
Chinese Journal of Nosocomiology
关键词
新生儿
病原菌
耐药性
Neonate
Pathogens
Drug resistance
