摘要
采用作者实验室的ELISA双抗夹心法测定尿中微量血红蛋白（Hb），正常人早、中、晚尿中Hb均少于5μg／ml，病例组尿中Hb量为75．7μg／ml，具有显著性差异，尿中Hb量与RBC数呈正相关。该法灵敏度高，特异性强，操作简便可靠，克服了化学法干扰因素及某些化学药物对人体毒性作用的缺点。其线性范围5～320μg／ml，不同浓度CV值分别为3．2和4．9，不受尿中蛋白干扰。
The level of hemoglobin (Hb) in urine was determined hy ELISA-doubl-antibody method established by authors’laboratory. The average amounts of Hb of healthy people and pa-tients were<5μg/ml and 75.7 μg/ml, respectively, and there was a significant difference be-tween the two groups. The Hb amount was positively related with RBC amount in urine. The lin-ear range of ELISA-double-antibody method was 5-320 μg/ml and the CV values were 3.2-4.9 without influence of urinary proteins. Some charateristics of the method are as follows: high sensitivity, strong specificity, simplicity, reliability and avoidance of toxicity of other chemical fac-tors or agents.
