摘要
生长激素刺激培养新生大鼠心肌细胞，用含ＭＢＰ凝胶分离法测定细胞外信号调节酶（ＥＲＫ）活性。用ＷｅｓｔｅｒｎＢｌｏｏｔｉｎｇ法检测磷酸化的ｐ３８ＭＡＰＫ，观察心肌细胞内ＭＡＰＫ超家族活性变化，探讨心肌细胞ＧＨ信号传导途径。结果发现：生长激素激活心肌细胞ＥＲＫｓ呈时间和浓度依赖性关系。而对ｐ３８ＭＡＰＫ无磷酸化激活作用。表明心肌生长激素信号传导通过ＭＡＰＫ超家族成员ＥＲＫ途径，而与ｐ３８ＭＡＰＫ途径无关。生长激素诱导的心肌细胞ＥＲＫ激活，可能是生长激素心脏效应的重要机制之一。
The cultured cardiac myocytes of neonatal rats were stimulated by growth hormone (GH),and then the activity of extracellular signal-regulated kinase(ERK) was assayed with the method of MBP-containing gel and the phosphorylated p38MAPK was detected with Western Blotting.The changes of MAPK superfamily activities were observed to explore GH signal transduction pathway in cardiac myocytes.The results showed that GH activated ERKs in cardiac myocytes in a time- and a dose-dependent manners, however,GH did not cause phospho-activation of p38MAPK in cardiac myocytes.This study indicates that GH signal transduction in cardiac myocytes is closely related to ERK,a member of MAPK superfamily,but is not relevant to p38MAPK pathway.GH-induced ERK activation in cardiac myocytes may be one of the important mechanisms by which GH affects the heart.
出处
《南通医学院学报》
1998年第3期296-298,共3页
ACTA Academiae Medicinae Nantong
基金
国家教委留学回国人员基金
日本文部省心血管研究基金
关键词
生长激素
P38MAPK
心肌细胞
信号传导
Growth hormone Extracellular signal-regulated kinase(ERK) p38MAPK Cardiac myocytes
