摘要
采用随机扩增多态性DNA(RAPD)技术,对东北地区杂草稻生态型及其与栽培稻杂交后代进行分子标记研究.所分析的10个随机引物共扩增出了93条DNA片段,其中多态性条带为36条,多态性比例为38.71%.结果表明,RAPD分子标记可以区别东北地区杂草稻、栽培稻及其杂交后代.
Throngh random amplified polymorphic DNA （RAPD） technology, studied the weedy rice ecotypes and hybrid rice cultivation of the northeastern region by the molecular marker. The 10 random primers amplified 93 DNA fragments, of which polymorphic bands is 36, polymorphism ratio is 38. 71%. The results showed that the RAPD molecular markers should difference the northeast Weedy rice, cultivated rice and their hybrids in northeast China.
出处
《延边大学农学学报》
2009年第2期105-108,共4页
Agricultural Science Journal of Yanbian University
基金
延边大学研究生科研基金项目
关键词
杂草稻
RAPD
分子标记
鉴别
Weedy rice
RAPD
molecular-marker
identification
