摘要
目的对滇西北地区分离的版纳病毒进行系统鉴定，明确与云南其他地区分离株的差异。方法对2005、2006年从滇西北地区分离的3株版纳病毒进行形态学、基因组带型和分子生物学鉴定，测定第12片段核苷酸序列，进行序列的同源性和系统进化分析。结果从2005、2006年滇西北蚊虫标本中分离到的3株版纳病毒为直径约70nm、无包膜、有双层衣壳的球形颗粒，基因组带型为6-6分布。3株版纳病毒间第12片段核苷酸序列同源性为99％，与云南其他地区和印度尼西亚版纳病毒毒株的最大同源性分别为98％和90％；系统进化分析显示滇西北分离株和云南其他地区分离株共同形成一个分枝；第12片段编码的氨基酸序列比较发现我国版纳分离株和印度尼西亚毒株间存在共同的氨基酸残基差异。结论首次从滇西北地区蚊虫中分离到3株版纳病毒，基因序列分析显示滇西北分离株和云南其他地区分离株同源性较高。
Objective To identify the Banna viruses isolated in northwestern part of Yunnan province in order to make the difference clear between the isolates and other Banna viruses isolated in other parts of Yunnan. Methods Three isolates of Banna virus isolated in 2005 and 2006 were identified by morphology, RNA-PAGE profile and molecular biologic method. Nucleotide and amino acid sequences of segment 12 of the 3 isolates were sequenced and analyzed. Results Three Banna viruses were isolated from mosquitoes collected in northwestern part of Yunnan during 2005 and 2006. Electron microscopy study showed that they are spherical with a diameter of 70 nm, no envelope but two layers of capsid. It was found that the genome of the 3 isolates composes of 12 segments presenting band profile of 6-6 in RNA-PAGE. Nucleotide acid sequence analysis about segment 12 showed that the identity was 99% between the 3 new isolates, 98% and 90% between the 3 isolates and the strains isolated in other parts of Yunnan, China and Indonesia, respectively. Phylogenetic analysis based on segment 12 gene showed that 3 new isolates clustered in the same branch with the viruses isolated in other parts of Yunnan. The same difference of amino acids was found between Banna viruses isolated in China and Indonesia strains in the analysis of segment 12. Conclusion Banna virus strains were firstly isolated from mosquitoes collected in northwestern part of Yunnan province. Nucleotide acid sequence analysis of the 3 new isolates showed higher identity with strains isolated in other parts of Yunnan.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2009年第6期495-498,共4页
Chinese Journal of Microbiology and Immunology
基金
国家科技部重大传染病专项资助项目（2003BA12A0801）
