摘要
目的探讨神经生长因子(NGF)基因修饰的骨髓间充质干细胞(BMSCs)对血管性痴呆(VaD)大鼠海马区细胞凋亡的影响。方法以NGF和(或)绿色荧光蛋白基因转染BMSCs;两血管法制备VaD模型。将大鼠随机分为假手术组、PBS组、BMSCs组及NGF修饰组。造模1周后,尾静脉注射NGF基因修饰的BMSCs;4周后行Mor-ris水迷宫检测,观察其行为学改变;末端脱氧核苷酰基转移酶介导性dUTP切口末端标记法检测海马凋亡细胞,免疫组化法检测大鼠海马区神经细胞NGF、N-甲基-D-天门冬氨酸受体1(NMDAR1)表达。结果与PBS组、BMSCs组比较,NGF修饰组逃避潜伏期明显缩短,海马区神经元凋亡率明显下降,NMDAR1表达明显降低(P<0.05或<0.01)。结论NGF基因修饰的BMSCs对VaD大鼠的学习记忆能力有一定改善作用;对其海马细胞有一定保护作用;可降低VaD大鼠海马区NR1表达,提高NGF表达。
Objective To study the effects of transplanting NGF gene modified bone marrow mesenehymal stem cells on the brains of rats with vascular dementia. Methods BMSCs were transfected with NGF or GFP gene. VaD models were randomly divided into four groups, namely sham group, PBS group, BMSCs group, NGF group. 4 Weeks later, behavioral abnormalities were observed by Morris water maze test and the expressions of NGF and NMDAR1 in neurons of hippocampus were detected by immunohistochemistry technique. TUNEL was used to identify apoptotic cells in hippocampi. Results Morris water maze test showed that average escape latency in the NGF group was shorter than that in the PBS group and the BMSCs group; the expression of NMDAR1 was significantly lower than those in the PBS group and BMSCs group （P 〈0. 01）. The apoptosis of nerve cell in NGF group was significantly less than that in the PBS group and BMSCs group （P〈0. 05）. Conclusions The injection of NGF gene modified BMSCs could improve learning and memory ability of VaD rats, reduce apoptosis in hippocampus, increase NGF expression and reduce the NR1 expression in rats with VaD.
出处
《山东医药》
CAS
北大核心
2009年第21期3-5,共3页
Shandong Medical Journal
基金
天津市科技发展计划项目(06YFSZSF01700)
关键词
间充质干细胞
骨髓
痴呆
血管性
细胞凋亡
mesenehymal stem cells, hone marrow
dementia, vascular
cell apoptosis
