摘要
目的:建立缬草抗心律失常有效部位的指纹图谱。方法:采用高效液相色谱法。色谱柱为Agilent C18色谱柱(250mm×4.6mm,5μm);流动相为乙腈-水溶液,梯度洗脱;检测波长:218nm;柱温:38℃;流速:1.0mL/min。结果:建立了缬草抗心律失常有效部位的HPLC-UV指纹图谱,标定了15个共有指纹峰,确定了其相对保留时间和相对峰面积的范围。结论:该方法准确、可靠,可为缬草的质量控制提供参考。
Objective： To establish HPLC fingerprints of the Antiarrhythmic fraction of Valeriana officinalis. Methods： Agilent C18 （250 mm× 4. 6 mm,5 μm） column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode. The column temperature was 380℃ and the detection wavelength was 218 nm. The detection time was 70 rain,and the flow rate was 1.0 mL/ min. Results ： Fifteen characteristic peaks were indicated in HPLC fingerprints. The relative retention time and the ranges of relative are- as of the common peaks were also determined. Conclusion ： This method is simple and accurate with a good reproducibility and provides a reference standard for the quality control of Valeriana officinalis.
出处
《中药材》
CAS
CSCD
北大核心
2009年第6期866-870,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(30500053)
关键词
缬草
指纹图谱
抗心律失常
Valeriana officinalis L.
Fingerprints
Antiarrhythmia
