摘要
从发病的暗纹东方鲀体内分离、鉴定了一株致病性嗜水气单胞菌,测定其毒力,命名为NT-1。以NT-1为抗原,免疫28周龄的罗曼蛋鸡,用水稀释脱脂处理,聚乙二醇(PEG)一步法制备高效价的卵黄抗体,微量凝集试验、ELISA检测其效价及特异性;不同浓度卵黄抗体的粗提液与NT-1菌液混合注射ICR小鼠,检测特异性卵黄抗体的抑菌效果。结果表明,微量凝集试验测得的特异性抗体效价达1∶16以上,ELISA效价达1∶12 800以上;高效价、特异性的卵黄抗体可维持90 d,特异性卵黄抗体ELISA效价达1∶640时对感染NT-1的ICR小鼠的保护率达到了100%。
The bacteria strain NT-1 was isolated from Takifugu obscurus and which were identified as Aeromonas hydrophila by bacteriological method. Hens were immunized with the bacteria. One-step polyethylene glycol （PEG-8000） precipitation procedure was used to purify the immunoglobulin Y （IgY）. The titres of purified IgY were detected by Microagglutination test and Enzyme-linked Immunosorhent Assay （ELISA）. The titer of Microagglutination in the IgY was higher than 1：16. The ELISA titers in the purified IgY was higher than 1：12 800. High titers IgY to Aeromonas hydrophila could be induced by immunization successfully, the specific activity last more than 90 days. When the ELISA titer in the IgY was higher than 1：640, its protection rate to the infected ICR mice was 100%.
出处
《湖北农业科学》
北大核心
2009年第6期1443-1446,共4页
Hubei Agricultural Sciences
基金
南通市农业科技创新项目(AL2007032)
关键词
嗜水气单胞菌
卵黄抗体
保护率
Aeromonas hydrophila
hen egg yolk immunoglobulin （IgY）
protection rate
