摘要
目的观察活血化瘀法对妊娠晚期大鼠宫颈成熟度的影响。方法选用妊娠18dSD大鼠,分别灌服丹红促生汤低、中、高剂量,并与西药、生理盐水作对照。第20日处死大鼠,立即取宫颈组织,经特殊处理后进行免疫组织化学、核酸原位杂交测定。结果丹红促生汤各剂量组、催产素组按排列顺序显著提高宫颈组织中IGF-I、IGF-ImRNA的阳性表达,与模型组比较有显著差异;丹红促生汤各剂量组与催产素组比较无显著差异。结论丹红促生汤具有活血化瘀作用,可影响细胞因子的表达,起到促宫颈成熟的作用。
Objective： To observe the effect of promoting blood circulation and removing blood stasis on cervical ripeness in the third trimester of pregnancy rats. Methods： SD rats that were pregnant for 18d were chosen and divided into Danhongcusheng Decoction low dosage, middle dosage and high dosage group, and compared with oxytocin group and physiological saline group. On the 20th day, the rats were sacrificed to get cervical tissue immediately for determining immunohistochemistry and nucleic acid hybridization in situ after special treatment. Results： Every Danhongcusheng Decoction groups, oxytocin group enhanced the expression of IGF-Ⅰ and IGF-Ⅰ mRNA significantly, and there was obvious difference between them and physiological saline group. There was no obvious difference between every Danhongcusheng Decoction groups and oxytocin group. Conclusion： Danhongcusheng Decoction has a good function of promoting blood circulation and removing blood stasis. It can affect the delivery of cell factor and improve cervical ripeness.
出处
《中国中医急症》
2009年第7期1138-1139,共2页
Journal of Emergency in Traditional Chinese Medicine
关键词
妊娠晚期宫颈
IGF—Ⅰ
活血化瘀大鼠
Third trimester of pregnancy
Cervix
IGF-Ⅰ
Promoting blood circulation and removing blood stasis
Rats
