摘要
目的研究血管生成抑制剂TNP-470对人肝癌高转移细胞株MHCC97-H体外形成血管生成拟态的影响。方法TNP-470作用于MHCC97-H细胞,应用MTT试验、体外侵袭实验,检测MHCC97-H细胞增殖活性和侵袭力的变化。建立MHCC97-H细胞人工基底膜基质凝胶体外三维细胞培养模型,观察MHCC97-H细胞能否形成血管生成拟态以及TNP-470对其影响,应用扫描电镜、透射电镜观察细胞结构改变。结果TNP-470对MHCC97-H细胞增殖无显著抑制作用,可抑制其体外侵袭能力,明显抑制MHCC97-H细胞形成血管生成拟态,减少细胞表面的微绒毛和细胞间连接。结论人肝癌高转移细胞株MHCC97-H可形成血管生成拟态,TNP-470能抑制人肝癌细胞株MHCC97-H体外血管生成拟态形成。
Objective To investigate the effect of angiogenesis inhibitor TNP-470 on the vasculogenic mimicry in human hepatocellular carcinoma cell line MHCC97-H in vitro. Methods The effects of TNP-470 on proliferation and invasion of MHCC97-H cells were detected by MTT assay and invasion assay in vitro. MHCC97-H cells were tested for their ability to form vasculogenic mimicry and the effect of TNP-470 on vaseulogenic mimicry formation in three-dimensional culture containing matrigel. The ultrastructrue of MH- CC97-H cells was observed by scanning and transmission electron microscope. Results The proliferation of MHCC97-H cells was not significantly affected by TNP-470. TNP-470 significantly inhibited invasion and vasculogenic mimicry formation of MHCC97-H cells in vitro, and decreased the surface microvillus and cell junction. Conclusions Human hepatocellular carcinoma cell line MHCC97-H can form vascular-like structure. TNP-470 can inhibit vasculogenic mimicry formation of human hepatocellular carcinoma cell line MH- CC97-H in vitro.
出处
《肝胆外科杂志》
2009年第3期223-225,共3页
Journal of Hepatobiliary Surgery
基金
国家自然科学基金资助项目(30772097)
安徽省优秀青年科技基金资助项目(08040106818)
安徽省科技攻关重点计划项目基金资助项目(07010302193)
安徽省自然科学基金资助项目(070413073)
安徽省重点科研计划资助项目(07020304100)
安徽省第二批"115"产业创新团队资助项目(安徽省立医院"肝细胞癌转移复发研究团队")
关键词
血管生成抑制
肝细胞癌
血管生成拟态
Angiogenesis inhibitors
Hepatocelluar carcinoma
Vasculogenic mimicry
