摘要
Background and Objective Cancer metastasis is not only the malignant characteristics of lung cancer, but also the key cause of failure to cure and high mortality. It has been proved
Background and Objective Cancer metastasis is not only the malignant characteristics of lung cancer, but also the key cause of failure to cure and high mortality. It has been proved that lung cancer metastasis is a complex process involving multiple gene and steps. Study on lung cancer metastasis has been focused on molecular pathogenesis for a long time, and outcomes are achieved in gene （DNA） and transcription （mRNA） level. Nm23 gene discovered in recent years is considered as one of the most important tumor metastatic suppressive gene. It has been proved that a close correlation exists between the high metastatic rate and the low expression or alleic deletion of nm23-H1 gene at DNA, mRNA level. But little is known about the molecular mechanism of metastasis suppression by nm23-H1 and the change of protein expression. By the limitation of itself, genomics cannot explain the interaction between proteins or between proteins and other bio-molecules, so the emphasis is gradually shifting to proteome recently. The aims of this study are to explore what effects will be achieved after transfection ofnrn23-Hl gene into human high-metastatic large cell lung cancer cell line L9981 for proteome, what difference of proteome will be existd comparing the human high- and low-metastatic large cell lung cancer cell lines.
出处
《中国肺癌杂志》
CAS
2009年第6期479-480,共2页
Chinese Journal of Lung Cancer
基金
supported by the grants from the National Natural Science Foundation of China
Science Foundation of Sichuan University (No. 30070333 and No.200349)
关键词
肺癌
治疗
疗效
临床
Nm23-H1 gene
Human high-metastatic lung cancercell line
Proteome
Two dimensional gel electrophoresis
Differ-ential expression
