摘要
以合成的孔雀石绿-牛血清白蛋白(MG-BSA)为免疫原免疫Balb/c小鼠,利用杂交瘤细胞技术,建立1株稳定分泌孔雀石绿单克隆抗体的杂交瘤细胞5G6,其分泌的抗体亚类为IgG1,轻链为κ型。间接ELISA测定腹水效价达1.28×105。间接竞争ELISA(ciELISA)显示其对孔雀石绿的50%抑制浓度(IC50)为1.36ng/ml,除结晶紫(CV)外,与无色孔雀石绿(LMG)没有交叉反应。
Balb/c Mice were immunized with the malachite green （MG） coupled with bovine serum albumin, and a strain of hybridoma cell named 5G6, which can stably secret monoclonal antibody （McAb）, were obtained by cell hybridoma technology. The subclass of the McAb was IgGl, and the light chain was κ type. The ascites titer determined by indirect ELISA was 1.28 ×10^5, while the indirectly competitive ELISA showed that its IC50 to malachite green was 1.36 ng/ml. Except crystal violet （CV）, the McAb had no cross-reaction with leucomalachite green （LMG）.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第13期223-226,共4页
Food Science
关键词
孔雀石绿
单克隆抗体
酶联免疫
malachite green （MG）
monoclonal antibody （McAb）
enzyme-linked immune sorbent assay
