摘要
以建立测定叶绿素类产品中脱镁叶绿酸含量方法为主要目的,采用85%丙酮和乙醚萃取色素,经17%的盐酸溶液酸化后,用可见分光光度计测定667nm下样品的吸光值,计算出脱镁叶绿酸的含量。结果表明:脱镁叶绿酸浓度在0.13mg/L～127mg/L范围内具有良好的线性关系,回归方程为Y=0.0275X+0.0025,R2=0.9998。加样平均回收率为90.0%。
To establish a spectrophotometer determination method of pheophorbide for chlorophyll products.The pheophorbide were determined by spectrophotometry at wavelenth 667 nm. Results showed that there was a good linear relation in the range of 0.13 mg/L-127 mg/L. A standard curve was obtained and the regression equation was Y=0.027 5X＋0.002 5, R^2=0.999 8. The average recovery rate was 90.0 %.
出处
《食品研究与开发》
CAS
北大核心
2009年第7期110-112,共3页
Food Research and Development
基金
天津市科技发展计划项目(05SYSYGX25000)
关键词
大麦苗粉
脱镁叶绿酸
分光光度法
barley grass powder
pheophorbide
spectrophotometer method
