摘要
利用降落PCR的方法,从蓖麻基因组DNA中克隆毒蛋白A链基因的560bp片段;利用一步法将该基因的2个正义片段和2个反义片段分别与pB I-121质粒连接,构建含该基因的正义重复和反义重复表达载体,为利用共抑制技术抑制蓖麻毒蛋白A链基因表达的研究奠定基础。
To clone the RTA gene from genome DNA of Ricinus commol/Lunis L. by touch down PCR. The expected 560bp gene fragment was obtained. Two RTA sense fragment and two RTA antisense fragment was linked to the plasmid pBI-121 respectively by one step. The sense and antisense repeat expression vector were constructed. This result provided a foundation for further research of RTA gene expression by co-suppression technology.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2009年第2期167-172,共6页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基因项目(30760123)
