摘要
目的探讨大鼠心肌梗死(MI)后不同时间,梗死组织基质细胞衍生因子-1(SDF-1)的表达以及与骨髓间充质干细胞(MSC)迁移的关系,为细胞移植提供可靠依据。方法在MI后不同时间,用PCR测定梗死组织中SDF-1表达的水平。提取梗死心肌组织提取液,在Costar Transwell双层细胞培养皿观察MI后不同时间梗死心肌组织对MSC迁移能力的影响。结果SDF-1在组织脏器内的表达存在差异,MI梗死心肌组织中SDF-1的表达呈现出时间变化的趋势,梗死后48h达到高峰水平。梗死后的心肌组织对MSC迁移的影响与SDF-1的表达呈相同的变化程序,梗死后48h对MSC的趋化作用最强。经SDF-1的受体CXCR4特异性阻滞剂处理后,MSC向梗死组织提取液迁移的能力受到明显抑制。结论MI后的早期,局部组织中SDF-1的表达明显升高,并可在MI后2周内维持在一个相对高的水平,其对于移植细胞向梗死区域迁移具有重要的作用。
AIM ： To explore the expression of stromal cell-derived factor-1 （ SDF-1 ） in infarcted myocardial tissue in rats at different periods after myocardial infarction and its relationship to the migration of bone marrow mesenchymal stem cell （MSC） so as to provide reliable basis for cellular transplantation. METHODS： SDF-1 expression was measured by RT-PCR in different organs and the infarcted hearts at different postoperative times. The migratory ability of MSC to the supernatants of the infracted area was evaluated through double-chamber transwell culture plates. RESULTS： Myocardial SDF-1 expression was significantly increased and peaked at the second day and remained at the high level for about 2 weeks. MSCs migrating toward infarcted myocardial tissue supernatant showed the same time course as that of SDF-1 expression, and migrating MSCs were significantly inhibited by the inhibitor of CXCR4, the receptor of SDF-1. CONCLUSION： Myocardial SDF-1 expression increases in the early phase post-MI and remains at a high level for a short period, which may play an important role in chemoattracting the transplanted cells to the infarcted tissue.
出处
《心脏杂志》
CAS
2009年第4期471-474,479,共5页
Chinese Heart Journal
关键词
基质细胞衍生因子-1
心肌梗死
细胞移植
骨髓间充质干细胞
stromal cell-derived factor-1
myocardial infarction
cellular transplantation
bone marrow mesenchymal stem cell
