摘要
目的研究临床分离的耐甲氧西林金黄色葡萄球菌（MRSA）的SCCmec基因型及亚型并对其耐药性进行分析，为临床治疗和流行病学研究提供依据。方法应用PCR法检测50株MRSAmecA基因和SCCmec的基因型及亚型。用纸片扩散法进行药敏试验。结果50株MRSAmecA基因均为阳性。45株为SCCmecⅢ型，3株为SCCmecmA型，2株为SCCmecⅡ型。未见SCCmecⅠ和SCCmecⅣ型。携带SCCmecⅡ、SCCmecⅢ或SCCmecⅢA的菌株均为多重耐药株。结论50株MRSA为多重耐药菌株，以SCCmecⅢ型为主。
Objective To investigate the SCCmec genotyping, subtype and antimicrobial susceptibility tests in methicillin resistant staphylococcus aureus to guide the clinical treatment and provide the proof for molecular epidemiology. Methods To detect mecA gene and SCCmec genetyping and subtype in 50 MRSA by PCR. According to CLSI＇s guideline, antimicrobial susceptibility tests were performed with disk diffusion. Results All 50 MRSA had mecA genes. 45 strains were SCCmec Ⅲ types ; 3 strains were SCCmec Ⅲ A types ; 2 strains were SCCmec Ⅱ types. There were no SCCmec Ⅰ and SCCmecⅣ types. SCCmec Ⅱ , SCCmec Ⅲ and SCCmec Ⅲ A type strains were all muhiresistant. Conclusion 50 MRSA are all multiresistant. SCCmec Ⅲ are the main types.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第3期197-199,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
葡萄球菌
金黄色
药物耐受
细胞
聚合酶链反应
Staphylococcus aureus
Drug Resistance, bacterial
Polymerase Chain Reaction
